Martin Luther University Halle-Wittenberg

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Dr. Vincent Doublet

Postdoctoral Researcher (now at the University of Exeter, UK)

Research focus

  • Invertebrate host-parasite ecology and evolution
  • Infectious disease dynamics and virology
  • Molecular evolution
  • Animal mitochondrial DNA evolution and transcription

And see my profile on: Google scholar    / Researchgate    / Publons   

Professional Career

• 2016-present – Postdoc at the University of Exeter, Penryn Campus, UK

• 2013-2015 – Postdoc at the German Centre for Integrative Biodiversity Research (iDiv   ) in Leipzig, Germany

• 2013 – Teaching assistant at the Department of General Zoology at Martin-Luther-Universität Halle-Wittenberg, Germany

• 2011-2013 – Postdoc fellow at Martin-Luther-Universität Halle-Wittenberg, Germany

• 2009-2010 – Teaching assistant at the Department of Population Biology of the University of Poitiers, France

Academic degrees

• 2010 Ph.D. in molecular evolution, University of Poitiers, France

• 2006 M.Sc. in evolutionary biology, University of Rennes 1, France

• 2003 B.Sc. in biological sciences, University of Le Havre, France

Research interests

My research interests are in the understanding the relations between coevolving host and microorganisms. I work on invertebrate models across different types of interactions, from mutualism (mitochondria-host cell interaction) to parasitism (fungal and viral pathogens), using experimental approaches and next-gen sequencing.

Synthesis of transcriptome response of honeybees to fungal and viral pathogens:

My current project at sDiv consists on a synthesis of the transcriptomic response of honeybees to pathogen infections, using published and unpublished datasets. I explore the commonality in host responses, with the aim of understanding the role of pathogens in bee decline. To conduct this project, I organized two workshops (10th-11th October 2013; 28th-29th April 2014) where I invited bee scientists and bioinformaticians at sDiv, Leipzig. This project is coordinated by Prof. Robert J. Paxton (MLU Halle-Wittenberg) and Prof. Christina M. Grozinger (PennState University). Link to the project webpage: Trans-Bee   .

Ecology and evolution of host-pathogen interactions in the honeybee:

I am also interested in the dynamics of host-pathogen interactions in the honeybee, using both fungal (Nosema spp.) and RNA viruses. I am looking at the rapid adaptation of RNA viruses and their impact on honeybee decline. I also look at the impact of pathogen co-infections on host’s fitness and pathogen evolution and epidemiology. I also investigated the impact of pesticides on host-pathogen dynamics in the honeybee, and the effect of pesticide-pathogen interactions (additive, synergistic or antagonistic) on honeybee survival and colony decline.

Mito-genomics of Crustacean:

My PhD research was focused on the evolution of the mitochondrial DNA (mtDNA) of terrestrial isopods. This mtDNA present an atypical structure, including linear molecules, which is related to an exceptionally inherited heteroplasmy (co-occurrence of several mitotypes in the same individual), located on a transfer RNA gene, alloacceptor for two amino-acids. I studied the architecture, evolution and transcription of this exceptional mtDNA model.

Scientific services and activities

Ad hoc reviewer for funding agencies and several peer-reviewed journals: Bulletin of Insectology / Evolutionary Bioinformatics / Gene / Infection Genetics and Evolution / Journal of Apicultural Research / Journal of Insect Science / Journal of Shellfish Research / Molecular and Cellular Probes / Parasitology / PLoS ONE / Psyche: A Journal of Entomology / Viruses.

Member of COLOSS (honeybee research association: http://www.coloss.org/   ) and the French Microbiological Society (SFM).

Full publication list (peer-reviewed)

15. Natsopoulou ME, Doublet V and Paxton RJ (2015) European isolates of the Microsporidia Nosema apis and Nosema ceranae have similar virulence in laboratory tests on European worker honey bees. Apidologie, 47(1): 57-65. doi:10.1007/s13592-015-0375-9   

14. Doublet V, Ubrig E, Alioua A, Bouchon D, Marcadé I and Maréchal-Drouard L (2015) Large gene overlaps and tRNA processing in the compact mitochondrial genome of the crustacean Armadillidium vulgare. RNA Biology, 12(10): 1159-1168. doi:10.1080/15476286.2015.1090078    [Temporary free access at: http://www.tandfonline.com/eprint/QAvhQUJsNDnmJ3aNZJU2/full   ]

13. Natsopoulou ME, McMahon DP, Doublet V, Bryden J and Paxton RJ (2015) Interspecific competition in honeybee intracellular gut parasites is asymmetric and favours the spread of an emerging infectious disease. Proceedings of the Royal Society B, 282: 20141896. doi:10.1098/rspb.2014.1896    [Press coverage in The Guardian   ]

12. Doublet V, Natsopoulou ME, Zschieche L and Paxton RJ (2015) Within-host competition among the honey bees pathogens Nosema ceranae and Deformed wing virus is asymmetric and to the disadvantage of the virus. Journal of Invertebrate Pathology, 124: 31-34. doi:10.1016/j.jip.2014.10.007   

11. Doublet V, Labarussias M, de Miranda JR, Moritz RFA and Paxton RJ (2015) Bees under stress: sublethal doses of a neonicotinoid pesticide and pathogens interact to elevate honey bee mortality across the life cycle. Environmental Microbiology, 17(4): 969-983. doi:10.1111/1462-2920.12426   

10. Huang SK, Csáki T, Doublet V, Dussaubat C, Evans JD, Gajda AM, Gregorc A, Hamilton MC, Kamler M, Lecocq A, Muz MN, Neumann P, Özkirim A, Schiesser A, Sohr AR, Tanner G, Tozkar CÖ, Williams GR, Wu L, Zheng H and Chen YP (2014) Evaluation of cage designs and feeding regimes for honey bee in vitro experiments. Journal of Economic Entomology, 107(1): 54-62. doi:10.1603/EC13213   

9. Suraporn S, Natsopoulou ME, Doublet V, McMahon DP and Paxton RJ (2013) Nosema ceranae is not detected in honey bees (Apis spp.) of northeast Thailand. Journal of Apicultural Research, 52(5): 259-261. doi:10.3896/IBRA.1.52.5.13   

8. Doublet V, Helleu Q, Raimond R, Souty-Grosset C and Marcadé I (2013) Inverted repeats and genome architecture conversions of terrestrial isopods mitochondrial DNA. Journal of Molecular Evolution, 77(3): 107-118. doi:10.1007/s00239-013-9587-7   

7. Fries I, Chauzat M-P, Chen Y-P, Doublet V, Genersch E, Gisder S, Higes M, McMahon DP, Martín-Hernández R, Natsopoulou ME, Paxton RJ, Tanner G, Webster TC and Williams GR (2013) Standard methodologies for Nosema research. Journal of Apicultural Research, 52(1). doi:10.3896/IBRA.1.52.1.14   

6. Williams GR, Alaux C, Costa C, Csáki T, Doublet V, Eisenhardt D, Fries I, Kuhn R, McMahon DP, Medrzycki P, Murray TE, Natsopoulou ME, Neumann P, Oliver R, Paxton RJ, Pernal SF, Shutler D, Tanner G, Van der Steen JJM and Brodschneider R (2013) Standard methods for maintaining adult Apis mellifera in cages under in vitro  laboratory conditions. Journal of Apicultural Research, 52(1). doi:10.3896/IBRA.1.52.1.04   

5. Cordaux R, Pichon S, Ben Afia Hatira H, Doublet V, Grčve P, Marcadé I, Braquart-Varnier C, Souty-Grosset C, Charfi-Cheikhrouha F and Bouchon D (2012) Widespread Wolbachia infection in terrestrial isopods and other crustaceans. Zookeys, 176: 123-131. doi:10.3897/zookeys.176.2284   

4. Doublet V, Raimond R, Grandjean F, Lafitte A, Souty-Grosset C and Marcadé I. (2012) Widespread atypical mtDNA structure in isopods (Crustacea: Peracarida) related to a constitutive heteroplasmy in terrestrial species. Genome, 55(3): 234-244. doi:10.1139/g2012-008   

3. Doublet V, Souty-Grosset C, Bouchon D, Cordaux R and Marcadé I (2008) A thirty million year-old inherited heteroplasmy. PLoS ONE, 3(8): e2938. doi:10.1371/journal.pone0002938   

2. Rispe C, Kutsukake M, Doublet V, Hudaverdian S, Legeai F, Simon J-C, Tagu D and Fukatsu T (2008) Large gene family expansion and variable selective pressures for cathepsin B in aphids. Molecular Biology and Evolution, 25(1): 5-17. doi:10.1093/molbev/msm222   

1. Marcadé I, Cordaux R, Doublet V, Debenest C, Bouchon D and Raimond R (2007) Structure and evolution of the atypical mitochondrial genome of Armadillidium vulgare (Isopoda, Crustacea). Journal of Molecular Evolution, 65(6): 651-659. doi:10.1007/s00239-007-9037-5   

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